Purity
Greater than 85% as determined by SDS-PAGE.
Species
Mus musculus (Mouse)
Expression Region
2-154aa
Target Protein Sequence
GLSDGEWQLVLNVWGKVEADLAGHGQEVLIGLFKTHPETLDKFDKFKNLKSEEDMKGSEDLKKHGCTVLTALGTILKKKGQHAAEIQPLAQSHATKHKIPVKYLEFISEIIIEVLKKRHSGDFGADAQGAMSKALELFRNDIAAKYKELGFQG
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The region for expressing recombinant Mouse Mb (Myoglobin) contains amino acids 2-154. This Mb (Myoglobin) protein is theoretically predicted to have a molecular weight of 21.9 kDa. This Mb (Myoglobin) recombinant protein is manufactured in mammalian cell. The N-terminal 10xHis tag and C-terminal Myc tag was fused into the coding gene segment of Mb (Myoglobin), making it easier to detect and purify the Mb (Myoglobin) recombinant protein in the later stages of expression and purification.
Mouse myoglobin (Mb) is a globular protein encoded by the Mb gene. Like its human counterpart, mouse myoglobin is primarily expressed in muscle tissues, where it plays a crucial role in oxygen storage and transport. Myoglobin is a heme-containing protein, with a single heme group per molecule that binds oxygen, allowing for its storage and release within muscle cells. This facilitates efficient oxygen supply to working muscles during periods of increased activity. Myoglobin is an essential component of the oxygen homeostasis machinery in muscle tissues. Research on mouse myoglobin helps to understand its role in oxygen metabolism, muscle function, and its implications in various physiological processes.
