H Antibody

Datasheet

Code	CSB-PA102159XA01DLU
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) H Polyclonal antibody

Uniprot No.

Q02308

Target Names

Alternative Names

H antibody; CG5460 antibody; Protein hairless antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) H protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Is a potent antagonist of neurogenic gene activity during sensory organ development. The expression of distinct cell fates by the trichogen (shaft) / tormogen (socket) sister cell pair depends on the level of H activity. A certain threshold level of H activity is required, below which both sister cells adopt the tormogen fate.

Gene References into Functions

Internal ribosome entry site mediated translation of H protein during mitosis. PMID: 27713501
There are two potential MAPK phosphorylation sites within the H protein; mutation of the two sites had only minor effects on the biological activity of H, suggesting that they are not major targets of MAPK PMID: 25363277
Genes activated by Notch signaling at the dorsal-ventral boundary of wing disc fall into classes affected differently by the loss of kinase module subunits. The distinctions do not result from different requirements for Hairless. PMID: 21793099
we identify a site in Hairless that is crucial for binding Su(H) protein PMID: 21737682
Hairless (H) is a Runt-interacting molecule that functions during segmentation. H is important for maintenance of engrailed (en) repression. PMID: 21325629
modifiers of the rough eye phenotype resulting from overexpression of the Notch antagonist hairless in Drosophila PMID: 12124948
Hairless acts as an adaptor to recruit the corepressors Groucho and dCtBP to Suppressor of Hairless. H directly binds two corepressor proteins, Groucho (Gro) and dCtBP. PMID: 12154126
Two isoforms of the Notch antagonist Hairless are produced by differential translation initiation. The presence of either isoform throughout the cell cycle allows efficient inhibition of Notch-regulated cell proliferation. PMID: 12422020
Hairless has a second mode of repression that antagonizes Notch intracellular domain and is independent of Gro or CtBP binding. PMID: 16287856
Data show that Pros26.4 negatively regulates Hairless at the genetic and molecular level, providing a novel mechanism of positive regulation of Notch signalling. PMID: 16410550
Hairless is a central component of the regulation of the Notch signalling pathway in Drosophila, and is hence essential for cell differentiation and tissue development in the fly. PMID: 17362357

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Subcellular Location

Nucleus.

Tissue Specificity

During embryogenesis expression is primarily in endo- and mesodermal cell layers. Ovary, embryos, larval and pupal imaginal disks.

Database Links

KEGG: dme:Dmel_CG5460

STRING: 7227.FBpp0083322

UniGene: Dm.7139

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