Insulin is a peptide hormone produced primarily by pancreatic beta cells that plays a fundamental role in glucose homeostasis and metabolic regulation. This hormone promotes cellular glucose uptake, stimulates glycogen synthesis, and inhibits gluconeogenesis, making it essential for maintaining blood glucose levels within physiological ranges. Insulin signaling pathways are extensively studied in diabetes research, metabolic disorders, and endocrinology. Dysregulation of insulin production or sensitivity leads to various pathological conditions including type 1 and type 2 diabetes mellitus.
The Chicken Insulin ELISA kit (CSB-E13293C) is designed for quantitative measurement of insulin (INS) in Gallus gallus samples. This competitive assay works with serum, plasma, and tissue homogenates with a detection range of 3.12 μIU/mL to 50 μIU/mL and sensitivity of 0.59 μIU/mL. The assay requires 50-100 μL sample volume, operates at 450 nm detection wavelength, and can be completed within 1-5 hours, providing researchers with a reliable tool for insulin quantification in avian studies.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This chicken insulin ELISA kit has been used in avian research studies focused on metabolic and endocrine profiling in laying hens. The kit allows quantification of insulin levels in serum samples as part of biochemical analyses examining hormonal and inflammatory biomarkers. Research applications have included studies examining the relationships between insulin and other metabolic regulators in poultry models.
• Metabolic research: Measurement of insulin concentrations alongside glucose measurements and other metabolic parameters in laying hen studies
• Endocrine profiling: Multi-analyte serum biochemical analyses incorporating insulin measurement with growth factors, hormones, and bone metabolism markers
• Inflammatory biomarker studies: Analysis of insulin levels in conjunction with pro-inflammatory cytokines and other immune-related factors in avian models
• Longitudinal monitoring: Serial insulin measurements across experimental timepoints to track metabolic changes in poultry research
