GN Antibody

Code CSB-PA670360XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GN Polyclonal antibody
Uniprot No.
Target Names
GN
Alternative Names
GN antibody; EMB30 antibody; GBF3 antibody; MIZ2 antibody; VAN7 antibody; At1g13980 antibody; F16A14.20 antibody; F7A19.7ARF guanine-nucleotide exchange factor GNOM antibody; Pattern formation protein EMB30 antibody; Protein EMBRYO DEFECTIVE 30 antibody; Protein MIZU-KUSSEI2 antibody; Protein VASCULAR NETWORK 7 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana GN protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Activates the ARF proteins by exchanging bound GDP for free GTP. Plays a role in vesicular protein sorting. Acts as the major regulator of endosomal vesicle trafficking but is also involved in the endocytosis process. Could function redundantly with GNL1 in the retrograde Golgi to endoplasmic reticulum trafficking. Regulates vesicle trafficking required for the coordinated polar localization of auxin efflux carriers which in turn determines the direction of auxin flow. Mediates the sorting of PIN1 from endosomal compartments to the basal plasma membrane and the polarization of PIN3 to the bottom side of hypocotyl endodermal cells. Involved in the specification of apical-basal pattern formation in the early embryo and during root formation. Required for correct cell wall organization leading to normal cell adhesion during seedling development. Plays also an essential role in hydrotropism of seedling roots.
Gene References into Functions
  1. GNOM preferentially localizes to a subdomain of the Golgi apparatus and redefined a role of this organelle in regulating endosomal function. PMID: 25012191
  2. data confirm a role for GNOM in endoplasmic reticulum (ER)-Golgi trafficking and reveal that a GNL1/GNOM-mediated early secretory pathway selectively regulates PIN1 basal polarity establishment in a manner essential for normal plant development PMID: 25646449
  3. GNOM-dependent auxin transport could affect local auxin biosynthesis. PMID: 24453227
  4. Therefore, an unknown GNOM-mediated vesicle trafficking system may mediate root hydrotropism and phototropism independently of PIN trafficking. PMID: 24388525
  5. GNOM is required for the establishment of the auxin response maximum for lateral root (LR) initiation, probably through the regulation of local and global auxin distribution in the root. PMID: 23390202
  6. Data conclude that provascular expression of GNOM is vital for both apical and basal tissue organization, and that epidermal GNOM expression is required for radial-to-bilateral symmetry transition of the embryo. PMID: 21138974
  7. PID and GNOM antagonistically regulate PIN polarity and plant development. Interference with GNOM activity leads to dynamic PIN transcytosis between different sides of the cell. PMID: 20040538
  8. large ARF-GEFs of the GBF1 class perform a conserved role in endoplasmic reticulum-Golgi trafficking and secretion, which is done by GNL1 and GNOM in Arabidopsis PMID: 17653190
  9. Distinct N-terminal domain of GNOM mediates dimerization and in addition interacts heterotypically with two other conserved domains in vivo. PMID: 18203920
  10. Pharmacological or genetic interference with GNOM leads specifically to apicalization of basal cargoes such as PIN1. PMID: 18394892
  11. GNOM-mediated vesicular trafficking plays an essential role in hydrotropism of seedling roots in Arabidopsis. PMID: 19052151

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Subcellular Location
Cytoplasm, cytosol. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Soluble and partially membrane-bound.
Tissue Specificity
Stems, leaves, flowers, siliques, floral inflorescence and roots. Expressed in the whole plant (at the protein level).
Database Links

KEGG: ath:AT1G13980

STRING: 3702.AT1G13980.1

UniGene: At.9165

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