Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Uniprot No.

P09260

Research Area

Signal Transduction

Species

Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)

Source

E.coli

Expression Region

18-317aa

Target Protein Sequence

NKSYVTPTPATRSIGHMSALLREYSDRNMSLKLEAFYPTGFDEELIKSLHWGNDRKHVFLVIVKVNPTTHEGDVGLVIFPKYLLSPYHFKAEHRAPFPAGRFGFLSHPVTPDVSFFDSSFAPYLTTQHLVAFTTFPPNPLVWHLERAETAATAERPFGVSLLPARPTVPKNTILEHKAHFATWDALARHTFFSAEAIITNSTLRIHVPLFGSVWPIRYWATGSVLLTSDSGRVEVNIGVGFMSSLISLSSGPPIELIVVPHTVKLNAVTSDTTWFQLNPPGPDPGPSYRVYLLGRGLDMN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

40.8 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

To produce recombinant Varicella-zoster virus Envelope glycoprotein H (gH) in E. coli, the gene of interest (18-317aa of HHV-3 gH) is co-cloned into an expression vector with an N-terminal 10xHis-tag and C-terminal Myc-tag gene and transformed into E. coli cells. The bacteria are cultured under conditions that induce protein expression. After reaching sufficient growth, the cells are lysed to release the recombinant protein. The harvested proteins undergo affinity chromatography purification. The purity of the HHV-3 gH protein is confirmed using SDS-PAGE, exceeding 85%.

Varicella-zoster virus glycoprotein H (gH) is a 118-kDa type I transmembrane protein with a large ectodomain and a short cytoplasmic tail [1]. It is recognized as the major neutralization target of the Varicella-zoster virus, with a conformational neutralizing epitope [2]. Studies have shown that gH, along with glycoproteins gE and gB, is incorporated into the Varicella-zoster virus virion envelope [3]. The cytoplasmic tails of these glycoproteins contain functional motifs that mediate endocytosis [1][3]. Varicella-zoster virus glycoprotein H plays a significant role in viral entry, replication, and pathogenesis [4].

References:
[1] T. Pasieka, L. Marešová, & C. Grose, A functional ynki motif in the short cytoplasmic tail of varicella-zoster virus glycoprotein gh mediates clathrin-dependent and antibody-independent endocytosis, Journal of Virology, vol. 77, no. 7, p. 4191-4204, 2003. https://doi.org/10.1128/jvi.77.7.4191-4204.2003
[2] Y. Akahori, K. Suzuki, T. Daikoku, M. Iwai, Y. Yoshida, Y. Asanoet al., Characterization of neutralizing epitopes of varicella-zoster virus glycoprotein h, Journal of Virology, vol. 83, no. 4, p. 2020-2024, 2009. https://doi.org/10.1128/jvi.02097-08
[3] L. Marešová, T. Pasieka, E. Homan, E. Gerday, & C. Grose, Incorporation of three endocytosed varicella-zoster virus glycoproteins, ge, gh, and gb, into the virion envelope, Journal of Virology, vol. 79, no. 2, p. 997-1007, 2005. https://doi.org/10.1128/jvi.79.2.997-1007.2005
[4] B. Berarducci, M. Sommer, L. Zerboni, J. Rajamani, & A. Arvin, Cellular and viral factors regulate the varicella-zoster virus ge promoter during viral replication, Journal of Virology, vol. 81, no. 19, p. 10258-10267, 2007. https://doi.org/10.1128/jvi.00553-07

Target Background

Function

The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Following initial binding to host receptor, membrane fusion is mediated by the fusion machinery composed of gB and the heterodimer gH/gL. May also be involved in the fusion between the virion envelope and the outer nuclear membrane during virion morphogenesis.

Subcellular Location

Virion membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein. Host endosome membrane; Single-pass type I membrane protein.

Protein Families

Herpesviridae glycoprotein H family

Code	CSB-EP357614VAP
Abbreviation	Recombinant Varicella-zoster virus gH protein, partial
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Varicella-zoster virus Envelope glycoprotein H (gH), partial

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