Recombinant Bovine coronavirus Nucleoprotein (N) is produced in a mammalian cell expression system, which appears to ensure proper folding and post-translational modifications. The protein spans the complete sequence of 1-448 amino acids and includes a C-terminal 6xHis-tag for simplified purification and detection. Purity levels exceed 85% based on SDS-PAGE confirmation, making this product suitable for various research applications that require high-quality recombinant proteins.
The nucleoprotein of Bovine coronavirus likely plays a critical role in packaging and protecting viral RNA. It participates in forming the ribonucleoprotein complex, which appears essential for viral genome replication and transcription. This protein has become a key focus in research aimed at understanding coronavirus replication mechanisms and developing diagnostic tools.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Antibody Development and Validation Studies
This full-length recombinant bovine coronavirus nucleoprotein can function as an immunogen for generating monoclonal or polyclonal antibodies specific to BCoV N protein. The C-terminal 6xHis tag simplifies purification and immobilization for antibody screening assays. Researchers may apply this protein in ELISA-based antibody characterization studies to determine binding specificity and affinity. The mammalian expression system likely preserves proper protein folding, which might maintain conformational epitopes important for antibody recognition.
2. Protein-Protein Interaction Studies
The nucleoprotein can be applied in pull-down assays to identify cellular or viral proteins that interact with BCoV N protein during infection. Its C-terminal His-tag allows immobilization on nickel-affinity matrices for capturing potential binding partners from cell lysates or viral protein preparations. Co-immunoprecipitation experiments may help validate specific interactions identified through pull-down approaches. These studies could contribute to understanding the molecular mechanisms behind bovine coronavirus replication and pathogenesis.
3. Structural and Biochemical Characterization
This recombinant protein provides material for biophysical studies focused on characterizing the structural properties of bovine coronavirus nucleoprotein. Researchers might perform circular dichroism spectroscopy to analyze secondary structure content and thermal stability. The protein could also be used in analytical ultracentrifugation or dynamic light scattering experiments to examine oligomerization states and protein aggregation behavior. Such biochemical analyses may provide insights into the fundamental properties of this viral nucleoprotein.
4. Comparative Coronavirus Research
The recombinant BCoV nucleoprotein may function as a reference standard in comparative studies examining nucleoproteins across different coronavirus species. Cross-reactivity studies with antibodies raised against other coronavirus nucleoproteins could reveal conserved and variable epitopes. Sequence and structural comparisons using this purified protein alongside nucleoproteins from other coronaviruses might help identify evolutionary relationships and functional domains. This application supports broader coronavirus research initiatives and pandemic preparedness studies.
