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Recombinant Varicella-zoster virus Envelope glycoprotein E (gE)

Recombinant Varicella-zoster virus Envelope glycoprotein E (gE)

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Code	CSB-CF362630VAP
Size	US$2508
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Uniprot No.

P09259

Research Area

Others

Alternative Names

gE; ORF68; Envelope glycoprotein E; gE

Species

Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)

Source

in vitro E.coli expression system

Expression Region

31-623aa

Target Protein Sequence

SVLRYDDFHTDEDKLDTNSVYEPYYHSDHAESSWVNRGESSRKAYDHNSPYIWPRNDYDGFLENAHEHHGVYNQGRGIDSGERLMQPTQMSAQEDLGDDTGIHVIPTLNGDDRHKIVNVDQRQYGDVFKGDLNPKPQGQRLIEVSVEENHPFTLRAPIQRIYGVRYTETWSFLPSLTCTGDAAPAIQHICLKHTTCFQDVVVDVDCAENTKEDQLAEISYRFQGKKEADQPWIVVNTSTLFDELELDPPEIEPGVLKVLRTEKQYLGVYIWNMRGSDGTSTYATFLVTWKGDEKTRNPTPAVTPQPRGAEFHMWNYHSHVFSVGDTFSLAMHLQYKIHEAPFDLLLEWLYVPIDPTCQPMRLYSTCLYHPNAPQCLSHMNSGCTFTSPHLAQRVASTVYQNCEHADNYTAYCLGISHMEPSFGLILHDGGTTLKFVDTPESLSGLYVFVVYFNGHVEAVAYTVVSTVDHFVNAIEERGFPPTAGQPPATTKPKEITPVNPGTSPLLRYAAWTGGLAAVVLLCLVIFLICTAKRMRVKAYRVDKSPYNQSMYYAGLPVDDFEDSESTDTEEEFGNAIGGSHGGSSYTVYIDKTR
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

85.4 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Customer Reviews and Q&A

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■ Q&A

I would like to request the following product:
VZV (partially purified VZV extract of infected cell cultures (ROD strain)

Very nice to receive your inquiry. Recombinant Varicella-zoster virus Envelope glycoprotein E(gE)
CSB-CF362630VAP >> Cell free
Expression Region: 31-623aa, full length
Tag Info: N-terminal 10xHis-sumo-tag
Target Protein Sequence:

We could also provide fragment for this protein. The product details are as below.
CSB-YP362630VAP1 >> Yeast
CSB-EP362630VAP1 >> E. coli
CSB-BP362630VAP1 >> Baculovirus
CSB-MP362630VAP1 >> Mammalian cell
Expression Region: 31-538aa, Virion surface domain
Tag Info: Yeast: N-terminal 6xHis-tag; E. coli, Baculovirus, Mammalian cell: N-terminal 10xHis-tag and C-terminal Myc-tag.
Target Protein Sequence:

Target Background

Function

Envelope glycoprotein that binds to the potential host cell entry receptor IDE.; In epithelial cells, the heterodimer gE/gI is required for the cell-to-cell spread of the virus, by sorting nascent virions to cell junctions. Once the virus reaches the cell junctions, virus particles can spread to adjacent cells extremely rapidly through interactions with cellular receptors that accumulate at these junctions. Implicated in basolateral spread in polarized cells. In neuronal cells, gE/gI is essential for the anterograde spread of the infection throughout the host nervous system. Together with US9, the heterodimer gE/gI is involved in the sorting and transport of viral structural components toward axon tips.; The heterodimer gE/gI serves as a receptor for the Fc part of host IgG. Dissociation of gE/gI from IgG occurs at acidic pH. May thus be involved in anti-VZV antibodies bipolar bridging, followed by intracellular endocytosis and degradation, thereby interfering with host IgG-mediated immune responses.

Gene References into Functions

Expression and glycan processing of VZV gE were decreased after ATG5 knockdown. PMID: 24198400
gE gene sequences revealed a novel non-synonymous mutations in the e1 and c1 epitopes. PMID: 23757839
GE/gI interaction is critical for VZV neurovirulence. PMID: 20962081
data show that the nonconserved N-terminal region of varicella-zoster virus glycoprotein E is required for VZV tropism for T cells and skin, which is necessary for its life cycle in the human host PMID: 19966293
The precursor nonglycosylated gE form (73 kDa) found in the endoplasmic reticulum binds to Insulin-degrading enzyme. PMID: 19864391
C-terminal domain of varicella-zoster virus glycoprotein E plays a role in viral replication in vitro and skin and T-cell tropism in vivo. PMID: 15507627
HSV deleted for glycoprotein E is impaired for cell-to-cell spread, and VZV gE could not complement this activity. PMID: 19233447

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Subcellular Location

Virion membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein. Host cell junction. Host Golgi apparatus membrane; Single-pass membrane protein. Host endosome membrane; Single-pass membrane protein.

Protein Families

Alphaherpesvirinae glycoprotein E family

Database Links

KEGG: vg:1487709

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