Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Signal Transduction
Alternative Names
(DNA-directed RNA polymerase)
Species
Escherichia phage T7 (Bacteriophage T7)
Expression Region
274-509aa
Target Protein Sequence
PPKPWTGITGGGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAINIAQNTAWKINKKVLAVANVITKWKHCPVEDIPAIEREELPMKPEDIDMNPEALTAWKRAAAAVYRKDKARKSRRISLEFMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYYWLKIHGANCAGVDKVPFPERIKFIEENHENIMACAKSPLENTWWAEQDSPF
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The expression region of this recombinant Escherichia phage T7 (Bacteriophage T7) T7 RNA polymerase covers amino acids 274-509. The expected molecular weight for the T7 RNA polymerase protein is calculated to be 34.6 kDa. This T7 RNA polymerase recombinant protein is manufactured in e.coli. The T7 RNA polymerase coding gene included the N-terminal 10xHis tag and C-terminal Myc tag, which simplifies the detection and purification processes of the recombinant T7 RNA polymerase protein in following stages of expression and purification.
The Escherichia phage T7 RNA polymerase (T7 RNAP) is an enzyme encoded by the T7 bacteriophage genome. It is a single-subunit RNA polymerase that recognizes and transcribes genes under the control of T7 promoter sequences. T7 RNAP is highly specific and efficient, making it a valuable tool in molecular biology research, particularly for in vitro transcription of RNA. Its use is prevalent in various applications, such as synthesizing RNA probes, producing RNA for structural studies, and generating messenger RNA (mRNA) for recombinant protein expression. T7 RNAP has become a standard component in molecular biology laboratories for its robustness and utility in diverse experimental techniques.
