Phospho-AR (Ser650) Antibody

Datasheet
Code CSB-PA831238
Product Type Polyclonal Antibody
Former Code(s) CSB-PA289177
Size US$360
Uniprot No. P10275
Image
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Androgen Receptor (Phospho-Ser650) Antibody (left) or the same antibody preincubated with blocking peptide (right).
  • Western blot analysis of extracts from 293 cells, treated with PMA or calf intestinal phosphatase (CIP), using Androgen Receptor (Phospho-Ser650) Antibody.
Immunogen Peptide sequence around phosphorylation site of serine 650 (T-T-S(p)-P-T) derived from Human Androgen Receptor.
Raised in Rabbit
Species Reactivity Human,Mouse
Specificity The antibody detects endogenous level of AndrogenReceptor only when phosphorylated at serine 650.
Tested Applications ELISA,WB,IHC;WB:1:500-1:1000,IHC:1:50-1:100
Relevance

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described.

Brinkman, A.O. et al. (1999) J. Steroid. Biochem. Mol. Biol. 69, 307-313.
Avila, D.M. et al. (2001) J. Steroid. Biochem. Mol. Biol. 76, 135-142.
Montgomery, J.S. et al. (2001) J. Pathol. 195, 138-146.

Form Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Clonality Polyclonal
Alias ANDR; DHTR; AR
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Target Names AR
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
Involvement in disease Androgen insensitivity syndrome (AIS); Spinal and bulbar muscular atrophy X-linked 1 (SMAX1); Androgen insensitivity, partial (PAIS)
Subcellular Location Nucleus, Cytoplasm
Protein Families Nuclear hormone receptor family, NR3 subfamily
Tissue Specificity Isoform 2 is mainly expressed in heart and skeletal muscle (PubMed:15634333). Isoform 3 is expressed by basal and stromal cells of prostate (at protein level) (PubMed:19244107).
Database Links

HGNC: 644

OMIM: 300068

KEGG: hsa:367

STRING: 9606.ENSP00000363822

UniGene: Hs.76704

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