Recombinant Severe acute respiratory syndrome coronavirus 2 Nucleoprotein (N) (D103Y) (Active)

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Code CSB-EP3325GMY(M5)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized N (CSB-EP3325GMY(M5)) at 2 μg/ml can bind N Mouse Monoclonal Antibody(CSB-MA33255A2m), the EC50 of N Mouse Monoclonal Antibody is 4.620-6.350 ng/ml. Biological Activity Assay
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized N (CSB-EP3325GMY(M5)) at 2 μg/ml can bind N Recombinant Antibody(CSB-RA33255A1GMY), the EC50 of N Recombinant Antibody is 7.358-9.976 ng/ml. Biological Activity Assay
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Product Details

Purity
Greater than 94% as determined by SDS-PAGE.
Activity
①Measured by its binding ability in a functional ELISA. Immobilized N (CSB-EP3325GMY(M5)) at 2 μg/ml can bind N Mouse Monoclonal Antibody(CSB-MA33255A2m), the EC50 of N Mouse Monoclonal Antibody is 4.620-6.350 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized N (CSB-EP3325GMY(M5)) at 2 μg/ml can bind N Recombinant Antibody(CSB-RA33255A1GMY), the EC50 of N Recombinant Antibody is 7.358-9.976 ng/ml.
Target Names
Uniprot No.
Research Area
Microbiology
Alternative Names
N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
Species
Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Source
E.coli
Expression Region
1-419aa(D103Y)
Target Protein Sequence
MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKYLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
Mol. Weight
49.8 kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
Subcellular Location
Virion. Host cytoplasm.
Protein Families
Betacoronavirus nucleocapsid protein family
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