Product Details

Uniprot No.

O43561

Target Names

LAT

Alternative Names

LAT; Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein; pp36; p36-38

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human Phospho-LAT (Y191)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2E4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200
IP	1:200-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Linker for activation of T-cells (LAT) serves as a critical adaptor protein in T cell receptor signaling, where its phosphorylation at tyrosine 191 creates docking sites for downstream effectors that drive T cell activation and immune responses. Detecting this specific phosphorylation event provides researchers with a direct readout of proximal TCR signaling activity, making it valuable for studies of immune cell function, signaling pathway dynamics, and immunological disorders.

This recombinant monoclonal antibody, generated against a synthetic phosphopeptide corresponding to human phospho-LAT at Y191, offers the reproducibility that phospho-specific detection demands. Because recombinant production ensures a sequence-defined, clonally derived reagent, researchers can expect consistent performance across experiments and between lots—particularly important when tracking subtle changes in phosphorylation status across experimental conditions.

Validation studies demonstrate reliable detection of phospho-LAT at the expected 38 kDa molecular weight in Western blot applications, with positive results obtained in Jurkat, HeLa, and HepG2 cell lysates following appropriate stimulation with EGF or pervanadate treatment. The antibody performs effectively in immunohistochemistry, where testing in paraffin-embedded human tonsil and colon cancer tissues shows clear staining using citrate buffer antigen retrieval on automated Leica Bond systems. Immunofluorescence applications have been validated in HeLa cells, revealing subcellular localization patterns, while immunoprecipitation capability has been confirmed in A549 cell lysates.

With verified performance across Western blot, immunohistochemistry, immunofluorescence, and immunoprecipitation platforms, this antibody provides flexibility for researchers investigating T cell signaling mechanisms, immune checkpoint biology, or phosphorylation-dependent protein interactions in immunology research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.

Gene References into Functions

Plasma membrane LAT activation precedes vesicular recruitment defining two phases of early T-cell activation. PMID: 29789604
LAT1 overexpression represents a negative prognostic marker that has been linked to tumor grade, proliferating potential and angiogenesis and mediates intracellular transport of anticancer agents. PMID: 28815339
The dimerization-dependent, cooperative binding of the growth factor receptor-bound protein 2 family to LAT may increase antigen receptor sensitivity by reducing signalosome formation at incompletely phosphorylated linker of activated T cells LAT molecules, thereby prioritizing the formation of complete signalosomes. PMID: 28951535
Nur77 suppresses CD4(+) T cell proliferation and uncover a suppressive role for Irf4 in TH2 polarization; halving Irf4 gene-dosage leads to increases in GATA3(+) and IL-4(+) cells. PMID: 28538176
LAT and phospholipase C-gamma dephosphorylation by SHP-1 inhibits natural killer cell cytototoxicity PMID: 27221712
Study examined molecular mobility within LAT:Grb2:SOS assemblies on supported membranes by single-molecule tracking. Trajectory analysis reveals a discrete temporal transition to subdiffusive motion below a characteristic timescale, indicating that the LAT:Grb2:SOS assembly has the dynamical structure of a loosely entangled polymer. PMID: 29045874
Here we report that phosphotyrosine-mediated assembly of adaptor protein LAT networks yields two distinct kinetic species of the Ras activator SOS PMID: 27370798
Overexpression of LAT is associated with microcephaly. PMID: 28965845
LAT and SLP-76 are randomly dispersed throughout the clusters that form upon T cell receptor engagement. PMID: 27875277
this study shows that inherited LAT deficiency should be considered in patients with combined immunodeficiency with T-cell abnormalities PMID: 27522155
This is the first report of a LAT-related disease in humans, manifesting by a progressive combined immune deficiency with severe autoimmune disease. PMID: 27242165
Data show that LAT1 plays an important role in regulating the uptake of essential amino acids such as leucine into endometrial cancer cells. Increased ability of BCH compared to LAT1 shRNA at inhibiting Ishikawa spheroid area suggests that other LAT family members may also contribute to cell growth. PMID: 27486861
Data indicate that the T cell-specific adaptor protein (TSAd) SH2 domain interacts with CD6 antigen and linker for activation of T cells protein (LAT) phosphotyrosine (pTyr) peptides. PMID: 27896837
High expression of LAT1 and ASCT2 correlates with metastasis and invasion in esophageal squamous cell carcinoma. PMID: 26936531
IFT20 is required for the delivery of the intracellular pool of LAT to the immune synapse in naive primary T lymphocytes. PMID: 26715756
Data show that the palmitoylation mutation of linker for activation of T cells (LAT)attenuated the signal transduction induced by glycosylphosphatidylinositol-anchored CD59 antigen in T cells. PMID: 26271970
HSV-1-encoded Us3 protein interrupted TCR signaling and interleukin-2 production by inactivation of the linker for activation of T cells. PMID: 25907557
Patients with severe aplastic anemia had increased levels of LAT and disturbed Th1-Th2 balance. PMID: 24673455
LAT is a modulator of CD3zeta and ZAP-70 tyrosine phosphorylation. PMID: 24204825
mutation of palmitoylation site of LAT-EGFP attenuated the signal transduction of CD59 in T cells PMID: 24200054
findings show that L-type amino acid transporter 1 is a major transporter for essential amino acids into activated human T cells PMID: 24038088
Histone hypoacetylation on LAT promoter could inhibit LAT expression and enhanced Th2 differentiation, while trichostatin A, a histone deacetylase inhibitor, promoted LAT expression and inhibited Th2 cytokine production PMID: 23360572
LAT is required to maintain calcium homeostasis in T cells. PMID: 22998346
A novel function and mechanism of TRAF6 action in the T cell receptor(TCR)-LAT signaling pathway indicates that LAT also plays an adapter role in TCR/CD28-induced activation of TRAF6. PMID: 23514740
LAT located at the cell surface can be recruited efficiently to activation-induced microclusters within seconds of T cell receptor (TCR) engagement. PMID: 23487428
LAT cleavage may act as a regulator of TCR-mediated activation of T-cells and not only as a transducer of cell death promoting stimuli. PMID: 23240581
Expression of constitutively active Raf transgene enhances lymphoproliferation, indicating a role for the Ras-MAPK pathway in LAT-mediated autoimmune hyperproliferation. PMID: 22984075
Nef employs a dual mechanism to disturb early TCR signaling by limiting the communication between LAT and SLP-76 PMID: 22802418
Modeling and simulation of aggregation of membrane protein LAT with molecular variability in the number of binding sites for cytosolic Grb2-SOS1-Grb2 PMID: 22396725
the segment comprising residues 112-126 of human LAT is required for its interaction with Lck. PMID: 22034845
Activation of T cell antigen receptors resulted in the recruitment and phosphorylation of linker for activation of T cells (Lat) from subsynaptic vesicles. Studies of Lat mutants confirmed that recruitment preceded Lat phosphorylation. PMID: 21642986
Data show that MAL regulates membrane order and the distribution of microtubule and transport vesicle docking machinery at the IS and, by doing so, ensures correct protein sorting of Lck and LAT to the cSMAC. PMID: 21508261
PECAM-1-mediated inhibition of GPVI-dependent platelet responses result from recruitment of SHP-2-p85 complexes to tyrosine-phosphorylated PECAM-1, which diminishes the association of PI3K with activatory signaling molecules Gab1 and LAT PMID: 20723025
LAT recruits Src homology 2 domain-containing leukocyte protein of 76 kDa (SLP-76) following T-cell receptor ligation and Akt kinase phosphorylation in Jurkat cells, activating the phosphoinositide 3-hydroxykinase (PI3K) signaling cascade. PMID: 21282515
Elevated expression of LAT1 is associated with squamous cell carcinoma of the lung. PMID: 19068093
Results indicate for the first time that LAT promotes TCR signal initiation and suggest that this adaptor may contribute to maintain active Lck in proximity of their substrates. PMID: 21152094
LAT ubiquitylation is a molecular checkpoint for attenuation of T-cell signaling. PMID: 21282648
These results reveal a novel role of HSP90 as a positive regulator for expression of LAT gene in activated T cells. PMID: 21251717
The adaptor LAT contains several conserved serine-based motifs, which are essential for proper signal transduction through the TCR. PMID: 20940326
Studies indicate that LAT is present in membrane rafts and involved in signalling through a number of receptors other than the T cell receptor. PMID: 20875087
A significant decrease of mRNA expression of LAT gene in T cells of asthmatic patients may be due to the up-regulation of its upstream regulatory factors Lck and ZAP-70. PMID: 18683785
LAT displacement from lipid rafts is a molecular mechanism by which PUFAs inhibit T cell signaling PMID: 12029091
Phosphorylation of the linker for activation of T-cells by Itk promotes recruitment of Vav. PMID: 12186560
LAT has a role in signal transduction for T cell activation and is stably associated with plasma membrane rafts PMID: 12515827
interaction with open-active form of Lck in lipid rafts reveals a new mechanism for the regulation of Lck in T cells PMID: 12570875
examination of synergistic assembly in T cell plasma membrane domains PMID: 12646565
The T cell adapter molecule LAT is required for correct orientation of the microtubule-organizing center, as it moves toward the interface of the T cell and antigen-presenting cell. PMID: 12847255
Results reveal the basis for preferential recognition of specific LAT sites by Gads. PMID: 15029250
CD3/T cell receptor complex signaling to beta 1 integrins is defective in LAT-deficient Jurkat T cells, and can be restored with expression of wild-type LAT. PMID: 15100278
LAB resembled a LAT molecule unable to bind phospholipase C-gamma1. PMID: 15153499

Hide All

Involvement in disease

Immunodeficiency 52 (IMD52)

Subcellular Location

Cell membrane; Single-pass type III membrane protein. Note=Present in lipid rafts.

Tissue Specificity

Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).

Database Links

HGNC: 18874

OMIM: 602354

KEGG: hsa:27040

UniGene: Hs.632179

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Code	CSB-RA012767A191phHU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in:Jurkat whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate(treated with EGF or Pervanadate) All lanes: Phospho-LAT antibody at 2.9μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 38 KDa Observed band size: 38 KDa IHC image of CSB-RA012767A191phHU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-RA012767A191phHU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-RA012767A191phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). Immunoprecipitating Phospho-LAT in A549 whole cell lysate Lane 1: Rabbit control IgG(1μg)instead of CSB-RA012767A191phHU in A549 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA012767A191phHU(3μg)+ A549 whole cell lysate(1mg) Lane 3: A549 whole cell lysate (20μg)
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Phospho-LAT (Y191) Recombinant Monoclonal Antibody

Product Details

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Target Background