Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

POR

Uniprot No.

P16435

Alternative Names

CPR; CYPOR; Cytochrome p450 oxidoreductase; DKFZp686G04235; FLJ26468; NADPH Cytochrome P450 Reductase; NADPH dependent cytochrome P450 reductase; NADPH--cytochrome P450 reductase; NCPR_HUMAN; P450 (cytochrome) oxidoreductase; P450 Cytochrome Oxidoreductase; P450R; por

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

2-671aa

Target Protein Sequence

INMGDSHVDTSSTVSEAVAEEVSLFSMTDMILFSLIVGLLTYWFLFRKKKEEVPEFTKIQTLTSSVRESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEYDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGVKFAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFELGLGDDDGNLEEDFITWREQFWLAVCEHFGVEATGEESSIRQYELVVHTDIDAAKVYMGEMGRLKSYENQKPPFDAKNPFLAAVTTNRKLNQGTERHLMHLELDISDSKIRYESGDHVAVYPANDSALVNQLGKILGADLDVVMSLNNLDEESNKKHPFPCPTSYRTALTYYLDITNPPRTNVLYELAQYASEPSEQELLRKMASSSGEGKELYLSWVVEARRHILAILQDCPSLRPPIDHLCELLPRLQARYYSIASSSKVHPNSVHICAVVVEYETKAGRINKGVATNWLRAKEPVGENGGRALVPMFVRKSQFRLPFKATTPVIMVGPGTGVAPFIGFIQERAWLRQQGKEVGETLLYYGCRRSDEDYLYREELAQFHRDGALTQLNVAFSREQSHKVYVQHLLKQDREHLWKLIEGGAHIYVCGDARNMARDVQNTFYDIVAELGAMEHAQAVDYIKKLMTK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

102.9kDa

Protein Length

Partial of BC034277

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Enhance your research endeavors with our high-quality Recombinant Human POR protein. Specifically tailored for the study of signal transduction, this NADPH--cytochrome P450 reductase (CPR; P450R) protein is produced in an E. coli expression system to ensure reliability and efficiency in your experiments. The partial protein (2-671aa) based on BC034277 reference sequence allows for outstanding performance in a variety of research applications.

Equipped with an N-terminal GST-tag for effortless protein purification and detection, our Recombinant Human POR protein exhibits a purity greater than 90% as determined by SDS-PAGE. Available in both liquid and lyophilized powder forms, this premium protein provides flexibility and adaptability for your research needs. Choose our Recombinant Human POR protein for a dependable resource in the exploration of signal transduction pathways.

Customer Reviews and Q&A

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■ Q&A

We had an inquiry about CSB-RP062744h:
could you let us know the following information?
1) Buffer formulation
2) Preservative/Concentration
3) Supplied Form (Liquid or Lyophilized)”
I see on your website the following regarding the buffer, “Tris-based buffer,50% glycerol”. Is there any more information you can provide to us? I would like to confirm we receive this product in the lyophilized format as well.

Very nice to receive your inquiry.
1) Buffer formulation: 10 mM Tris-HCl,1 mM EDTA, pH 8.0, 50% glycerol
2) There contains NO Preservative.
3) The current lot is supplied in liquid form. The lyophilized form is also available if you would like, please remark this request in your PO.

Could you send a price to me for the following below. These will be used on the Biocore so if you can recommend a good purity version, that would be great.
500ug of Human BMP2

Thanks for your inquiry. I checked with our protein center and recommend these products for you.
500ug of Human BMP2
CSB-RP094194h Recombinant Human Bone morphogenetic protein 2(BMP2)
Expression system: E.coli
Expression region: 283-396aa; Full length of mature protein.
Tag information: N-terminal 6xHis-tagged
Target protein sequence:

QAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR

Lead time: 3-7 business days.

Target Background

Function

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

Gene References into Functions

Both CYP1A2 and CYP2B4 activities were affected by their coexpression in a manner consistent with formation of the high-affinity POR-CYP1A2-CYP2B4 complex. PMID: 29233819
genetic association studies in population in Scotland: data suggest, in type 2 diabetes treated with sulfonylureas, 2 SNPs in CYP2C9 (CYP2C9*2, R144C, rs1799853; CYP2C9*3, I359L, rs1057910) are associated with drug-induced hypoglycemia; an SNP in POR (POR*28, A503V, rs1057868) is associated with better response to sulfonylureas. (CYP2C9 = cytochrome P450 family 2 subfamily C member 9; POR = cytochrome p450 oxidoreductase) PMID: 28656666
The POR*28 heterozygote appears to be associated with the stable dose of acenocoumarol. PMID: 28592191
Younger age, POR*28 allele, and CYP3A5*3 allele were associated with higher cyclosporine dosing requirements and lower concentration/dose ratio. PMID: 29135906
Suggest that hepatic POR and P450s are coregulated, and that POR plays a complex role in P450-mediated metabolism. PMID: 27271371
A homodimer model can resolve the conundrum as to how cytochrome P450 oxidoreductase and cytochrome b5 compete for the same binding site on cytochrome P450c17. (Review) PMID: 28000554
Compound heterozygosity of a paternal submicroscopic deletion and a maternal missense mutation in POR gene: Antley-bixler syndrome phenotype in three sibling fetuses PMID: 26969897
analysis of mutations and polymorphisms in POR linked to metabolism of steroids and xenobiotics and pathology of P450 oxidoreductase deficiency (PORD) PMID: 27068427
No significant differences in the distribution of haplotypes between cases and controls were detected. In conclusion, this study reveals that four SNPs in the POR gene (rs3823884, rs3898649, rs239953 and rs17685) may affect the susceptibility of smoking cessation in a Chinese Han population. PMID: 27660057
analysis of the role of the active site loop in coenzyme binding and flavin reduction in cytochrome P450 reductase PMID: 27461959
Current studies demonstrate that, unlike previously described mutations, A287P causes POR deficiency disorder due to conformational instability leading to proteolytic susceptibility in vivo, rather than through an inherent flavin-binding defect. PMID: 27496950
population pharmacokinetic analysis identified that the combined genotype of CYP3A5-POR was the only covariant for the apparent clearance of tacrolimus PMID: 26227094
Case Report: delayed diagnosis of 46, XY disorder of sex development due to P450 oxidoreductase (POR) deficiency. PMID: 27376429
Interindividual variability in CYP2C19 activity is due to differences in both CYP2C19 protein content associated with gene diplotypes and the cytochrome P450 oxidoreductase content. PMID: 26323597
POR rs2868177 and CYP2B6*6 variants contribute to the interindividual variability in human CYP2B6 activity, which may affect the disposition and interaction of other CYP2B6 substrate drugs PMID: 27439448
For the first time, the SNP POR*37 831-35C>T was confirmed as a minor but statistically significant factor associated with inter-individual variation in warfarin maintenance dose in Han Chinese PMID: 27488389
POR*28 does not significantly influence Tac pharmacokinetic parameters. PMID: 26829596
the POR g.6593A>G polymorphism significantly influences CYP2B6 activity PMID: 26580670
P450 (Cytochrome) Oxidoreductase Gene (POR) Common Variant (POR*28) Significantly Alters CYP2C9 Activity in Swedish, But Not in Korean Healthy Subjects PMID: 26669712
POR A503V might decrease the risk of bladder cancer by reducing its metabolic activity. PMID: 26123203
POR besides CYP2B6 can influence cyclophosphamide metabolism PMID: 26544874
Study is a thorough structural, dynamic, and thermodynamic characterization of the soluble form of human microsomal CPR using small-angle x-ray scattering and nuclear magnetic resonance spectroscopy under various conditions PMID: 25809265
This study confirmed that CYP3A5*1 was associated with lower tacrolimus trough concentrations. POR*28 was associated with decreased tacrolimus trough concentrations although the effect was small possibly through enhanced CYP3A4 enzyme activity. PMID: 26067485
electron transfer between mCPR and aromatase PMID: 26087061
POR is the main determinant of SN30000 sensitivity in 3 different genetic backgrounds. Head and neck squamous cell carcinomas showed heterogeneous POR expression. There may be a relationship between HPV status and hypoxia-activated prodrug sensitivity. PMID: 26297733
We identified seven unreported SNP genetic variations. The p.Pro384Leu variant exhibited reduced enzymatic activities compared with wild-type. PMID: 25712184
Results show that combining the CYP3A5*1, POR*28 and CYP3A4*22 genotypes allows partial differentiation of early tacrolimus dose requirements and the time to reach therapeutic target concentrations after transplantation. PMID: 25322286
In children with FH, carriage of POR*28 allele is associated with reduced effect of atorvastatin on TChol and LDLc and therefore identifies FH children that may require higher atorvastatin doses to achieve full therapeutic benefits PMID: 25521355
Transient kinetic dissection of the reaction of POR with NADPH and the reduction in cytochrome c by POR using stopped-flow techniques revealed defects in individual electron transfer steps mediated by A287P. PMID: 25728647
Interactions of HO-2 with CPR and BVR, were evaluated. PMID: 25196843
Data shows that POR*28 allele was not associated with the lipid-lowering effect of atorvastatin. PMID: 24430948
results showed that the CYP2C19 and/or P450 oxidoreductase genotypes have an impact on the clearance of clobazam and/or N-desmethylclobazam PMID: 24345815
NADPH-cytochrome P450 reductase is regulated by all-trans retinoic acid and by 1,25-dihydroxyvitamin D3 in human acute myeloid leukemia cells. PMID: 24642534
The role of Cytochrome P450 reductase is to provide electrons to a redox partner, the interactions with cytochrome P450 2D6 (2D6) were investigated and a possible complex structure is suggested. PMID: 23832577
results show that FMO3 E158K and POR A503V polymorphisms are minor sources of nicotine metabolism variation, insufficient to appreciably alter smoking. PMID: 24448396
POR*28 allele is an important source of CYP2C9 activity variability and combined with CYP2C9 gene poly-morphisms may explain individual variability in the effect of sulfonylureas. PMID: 24464600
Seventeen SNPs of CYP3A5, CYP3A4, COMT, IL-10 and POR were identified. PMID: 24465960
Candidate-gene study shows that polymorphisms in PPARalpha and POR might predispose patients being treated with tacrolimus to the development of NODAT after kidney transplantation PMID: 24113216
Findings regarding the interaction of NADPH-P450 reductase (NPR) with cellular stress response (CSR) indicated function of NPR in hypoxic response. PMID: 24491563
Domain motion is linked closely to the individual steps of the catalytic cycle of cytochrome P450 reductase. PMID: 23911089
although POR expression is a potential biomarker of sensitivity to some HAP, identification of other one-electron reductases responsible for HAP activation is needed for their rational clinical development. PMID: 24196959
Structural, enzymatic and clinical implications of the NADPH P450 oxidoreductase mutations linked to newly identified disorders in humans, now categorized as POR deficiency[review]. PMID: 23353702
POR genetic variants affect CYP2C9 activities. The impact of a POR variant on catalysis varies with the isoform of CYP2C9 and the assay substrate. PMID: 22547083
Six novel NADPH-P450 oxidoreductase missense variants in Ashkenazi and Moroccan Jewish populations were identified. PMID: 22462747
The data suggested that cytochrome P450 reductase plays a major role in reactive oxygen species generation by liver cells under the basal conditions, but a minimal rike during simulated in vitro ischemia-reperfusion injury. PMID: 21973081
NADPH cytochrome P450 reductase prevents this translocation and promotes oligomerization of HO-1. PMID: 22545110
Studies indicate that electron transfer in cytochrome P450 reductase is conformationally gated. PMID: 21762695
Clinical, biochemical, and genetic findings in a large PORD cohort. PMID: 22162478
POR*28 C > T polymorphism increases 1-hydroxylation of midazolam after intravenous administration. The polymorphism is associated with increased hepatic, but not intestinal, CYP3A activity in healthy Chinese volunteers. PMID: 22177374
These data indicate that the POR*28 SNP is associated with additional increases in early tacrolimus dose-requirements in patients carrying a CYP3A5*1 allele. PMID: 21770725

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Involvement in disease

Antley-Bixler syndrome, with genital anomalies and disordered steroidogenesis (ABS1); Disordered steroidogenesis due to cytochrome P450 oxidoreductase deficiency (DISPORD)

Subcellular Location

Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side.

Protein Families

NADPH--cytochrome P450 reductase family; Flavodoxin family; Flavoprotein pyridine nucleotide cytochrome reductase family

Database Links

HGNC: 9208

OMIM: 124015

KEGG: hsa:5447

STRING: 9606.ENSP00000419970

UniGene: Hs.354056

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