Recombinant Murine coronavirus Nucleoprotein (N)

Code CSB-YP361047MJY
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP361047MJY
MSDS
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Source E.coli
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Code CSB-EP361047MJY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP361047MJY
MSDS
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Source Baculovirus
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Code CSB-MP361047MJY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
N
Uniprot No.
Alternative Names
N; 7a; Nucleoprotein; Nucleocapsid protein; NC; Protein N
Species
Murine coronavirus (strain A59) (MHV-A59) (Murine hepatitis virus)
Expression Region
1-454
Target Protein Sequence
MSFVPGQENA GGRSSSVNRA GNGILKKTTW ADQTERGPNN QNRGRRNQPK QTATTQPNSG SVVPHYSWFS GITQFQKGKE FQFAEGQGVP IANGIPASEQ KGYWYRHNRR SFKTPDGQQK QLLPRWYFYY LGTGPHAGAS YGDSIEGVFW VANSQADTNT RSDIVERDPS SHEAIPTRFA PGTVLPQGFY VEGSGRSAPA SRSGSRSQSR GPNNRARSSS NQRQPASTVK PDMAEEIAAL VLAKLGKDAG QPKQVTKQSA KEVRQKILNK PRQKRTPNKQ CPVQQCFGKR GPNQNFGGSE MLKLGTSDPQ FPILAELAPT VGAFFFGSKL ELVKKNSGGA DEPTKDVYEL QYSGAVRFDS TLPGFETIMK VLNENLNAYQ KDGGADVVSP KPQRKGRRQA QEKKDEVDNV SVAKPKSSVQ RNVSRELTPE DRSLLAQILD DGVVPDGLED DSNV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
Gene References into Functions
  1. Coronavirus N phosphorylation by GSK-3 allows recruitment of the RNA helicase DDX1, which facilitates template readthrough and enables longer viral positive-sense single-stranded genomic RNA synthesis. PMID: 25299332
  2. The second domain of N protein plays an essential role in recognizing the RNA structure that allows the selective packaging of genomic RNA into assembled virions. PMID: 24501403
  3. These results support a model in which the N-nsp3 interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection. PMID: 23760243
  4. Structures of the N- and C-terminal domains of MHV-A59 nucleocapsid protein PMID: 21203940
  5. analysis of the MHV-CoV A59 nucleocapsid protein COOH-terminal negative charges PMID: 17037518
  6. analysis of mouse hepatitis coronavirus nucleocapsid phosphorylation PMID: 17037524
  7. Two domains of N protein are critical for M protein recognition during virus assembly. PMID: 19420077

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Subcellular Location
Virion. Host endoplasmic reticulum-Golgi intermediate compartment. Host Golgi apparatus.
Protein Families
Betacoronavirus nucleocapsid protein family
Database Links

KEGG: vg:1489757

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