Greater than 90% as determined by SDS-PAGE.
Less than 1.0 EU/ug as determined by LAL method.
①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind Biotinylated Anti-SARS-CoV-2-S Antibody (CSB-RA33245D1GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 106.2-131.2 ng/ml. ②Measured by its binding ability in a functional ELISA. Immobilized human ACE2 (CSB-MP866317HU) at 2 μg/ml can bind SARS-CoV-2-S1-RBD, the EC50 of SARS-CoV-2-S1-RBD protein is 8.363-12.82 ng/ml.
S; 2; Spike glycoprotein; S glycoprotein; E2; Peplomer protein)
Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Target Protein Sequence
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
3-7 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
CUSABIO expressed the human SARS-CoV-2 spike glycoprotein (S) amino acid residues Arg319-Phe541 carrying a C-terminal 6xHis-mFc-tag in the mammalian cells. The obtained product is the recombinant partial-length human SARS-CoV-2 S protein. The purity of this protein was measured by SDS-PAGE and reached up to 90%. It migrated to a band with a molecular weight of 66 kDa on the gel under reducing conditions. And it contains less than 1.0 EU/ug endotoxin determined by the LAL method. Its bio-activity was tested through the functional ELISA. In-stock recombinant SARS-CoV-2 S protein is offered now. This S protein has been cited in one reference by Li Zhu et al.
SARS-CoV-2 has been threatening and hitting humans across the world since its emerging in late 2019. The S protein of the SARS-CoV-2 is responsible for receptor recognition, viral attachment, and entry into host cells.